DAF yields a cloned marker linked to the soybean (Glycine max) supernodulation nts-1 locus

Reference Report for SoyBase91001745

Title:	DAF yields a cloned marker linked to the soybean (Glycine max) supernodulation nts-1 locus
Authors:	Men, A.E., Gresshoff, P.M.
Source:	J. Plant Physiol. 2001, 158(8):999-1006
Abstract:	We report a further characterization of the genomic region containing the soybean supernodulation gene NTS-1. We performed a search for new markers linked to NTS-1 by combining DNA amplification fingerprinting (DAF) and bulked segregant analysis (BSA). The search resulted in one cloned polymorphism (B44-456) linked in trans, 8.5cM from the locus. Southern hybridization showed duplication of the B44-456 sequence in the soybean genome. Additionally, a DNA database search revealed one Arabidopsis thaliana genomic clone from chromosome I possessing 62% homology to the B44-456 marker. A relatively low number of polymorphisms were identified by several PCR marker technologies for this soybean genomic region, providing an additional support for its highly conserved and/or duplicated organization.

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