Reference Report for SoyBase8101930
Title: | Purification of blackgram mottle virus (BMoV) using magnesium-bentonite |
Authors: | Balasubrahmanyam, A., Kapoor, H.C., Varma, A. |
Source: | Phytochem. 1997, 44(7):1237-1240 |
Abstract: | Blackgram mottle virus was purified from French bean (Phaseolus vulgaris) cv. Contender tissue using two different methods involving differential centrifugation. The use of Mg-bentonite during purification produced a homogeneous and pure virus preparation. The Mr of RNA and coat protein were 1400 and 39k, respectively. The RNA was infectious at a concentration as low as 7 micrograms ml(-1). |