A species-specific DNA probe for the identification of Meloidogyne hapla

Reference Report for SoyBase52202812

Title:	A species-specific DNA probe for the identification of Meloidogyne hapla
Authors:	Dong, K., Dean, R.A., Fortnum, B.A., Lewis, S.A.
Source:	Nematropica 2001, 31(1):17-23
Abstract:	Here we report the development of a DNA probe specific to Meloidogyne hapla, among four species in the genus that we have studied. A small-insert genomic library from an isolate of M. hapla was constructed in the pBluescript vector. From the library, 3 200 clones were differentially screened against genomic DNA from M. arenaria, M. incognita and M. javanica. About 99% of the clones hybridized positively to the three nematode species. The clones that hybridized specifically to the genomic DNA from different M. hapla isolates were selected and further characterized by slot-blot and Southern blot analyses. Sequence analysis from one of the clones reveals a 171 bp A and T-rich DNA insert, and a 55-base DNA oligonucleotide specific to M. hapla isolates

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