Comparison of tobacco streak virus isolates from soybean and tobacco.

Reference Report for IND80067891

Title:	Comparison of tobacco streak virus isolates from soybean and tobacco.
Authors:	Ghanekar, A.M., Schwenk, F.W.
Source:	J. Phytopath. 1980, 97(2):148-155
Abstract:	Factors affecting component proportions, quality and quantity of the purified virus of 2 isolates of tobacco streak virus (TSV) designated A-TSV from soybean and W-TSV from tobacco were compared. Each isolate yielded 4-5 mg virus from 100 g host tissue. Rate-zonal density gradient centrifugation in sucrose solutions of both isolates showed top (non-infective), middle and bottom (both infective) nucleoprotein components. The proportion of each component varied according to the host, host part and season. Antiserum prepared against A-TSV had detectable amounts of antibodies to host proteins; W-TSV antiserum did not. Agar double-diffusion did not distinguish between A-TSV and W-TSV and they did not differ from type strains HF-TSV or RN-TSV. However, A-TSV and W-TSV are different from each other. Infective A-TSV was purified from immature soybean seed embryos and seed coats and from mature soybean seed embryos, but not from mature seed coats.

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA

U.S. Public Domain