Nucleotide sequence and E. coli expression of the coat protein gene of the yellowing strain of soybean dwarf luteovirus.

Reference Report for IND20398437

Title:	Nucleotide sequence and E. coli expression of the coat protein gene of the yellowing strain of soybean dwarf luteovirus.
Authors:	Smith, O.P., Damsteegt, V.D., Harris, K.F., Vonder Haar, R.
Source:	Arch. Virol. 1993, 133(1-2), 223-231
Abstract:	The nucleotide sequence of the coat protein gene of the yellowing (Y) strain of soybean dwarf virus (SbDV) was determined from cloned cDNA. The gene contains 600 nucleotides and encodes a protein of 200 amino acids with a calculated M(r) of 22200. The identity of the open reading frame (ORF) encoding the coat protein was confirmed by expression of an Escherichia coli beta-galactosidase fusion protein and detection by a dot blot immunoassay. Sequence comparisons of the deduced coat protein amino acids to several luteoviruses demonstrated that the SbDV-Y coat protein ORF shares greatest similarity with bean leaf roll virus (BLRV) (65%).

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