Genome-wide identification of ascorbate-glutathione cycle gene families in soybean (Glycine max) reveals gene duplication events

Reference Report for AP20210726.1

Title:	Genome-wide identification of ascorbate-glutathione cycle gene families in soybean (Glycine max) reveals gene duplication events
Authors:	Costa, H. J., Roque, A. L. M. , Aziz, S., dos Santos, C. P., Germano, T. A., Batista, M. C., Thiers, K. L. T., Saraiva, K. D. C., Arnholdt-Schmitt, B.
Source:	Costa et al. 2018 BIOMAC
Abstract:	Ascorbate-glutathione (AsA-GSH) cycle plays an important role in tuning beneficial ROS accumulation for intracellular signals and imparts plant tolerance to oxidative stress by detoxifying excess of ROS. Here, we present genome-wide identification of AsA-GSH cycle genes (APX,MDHAR,DHAR, andGR) in several leguminous species and expression analyses inG. maxduring stress, germination and tissue development. Our data revealed 24 genes inGlycinegenus against the maximum of 15 in other leguminous species, which was due to 9 pars of duplicated genes mostly originated from sub/neofunctionalization. CytosolicAPXandMDHARgenes were highly expressed in different tissues and physiological conditions. Germination induced genes encoding AsA-GSH proteins from different cell compartments, whereas vegetative phase (leaves) stimulated predominantly genes related to chloroplast/mitochondria proteins. Moreover, cytosolicAPX-1,2,MDHAR-1a,1bandGRgenes were the primary genes linked to senescence and biotic stresses, whilestAPX-a,bandGR(from organelles) were the most abiotic stress related genes. Biotic and abiotic stress tolerant genotypes generally showed increasedMDHAR,DHARand/orGRmRNA levels compared to susceptible genotypes. Overall, these data clarified evolutionary events in leguminous plants and point to the functional specificity of duplicated genes of the AsA-GSH cycle inG. max.

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA

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