Genome-wide identification, characterization and expression analysis of BES1 gene family in tomato

Reference Report for AP20210716.1

Title:	Genome-wide identification, characterization and expression analysis of BES1 gene family in tomato
Authors:	Su, D., Xiang, W., Wen, L., Lu, W., Shi, Y., Liu, Y., Li, Z.
Source:	Su et al. 2021 BMC Plant Biol., 21:161
Abstract:	Background As the key regulators in BR signaling,BES1family genes regulate thousands of target genes involved in various development processes. So far, the functions ofBES1family are poorly understood in tomato, and a comprehensive genomic and expressional analysis is worth to conduct for this family. Results Here, nineSlBES1family members were identified in tomato and classified into five groups based on the conserved motif, gene structure and phylogenetic analysis. Synteny among tomato,Arabidopsis, pepper and rice were further analyzed to obtain insights into evolutionary characteristics. Severalcis-elements related to hormone, stress and plant development were exhibited in the promoter regions ofSlBES1family genes. Subcellular localization showed seven members localized both in the nucleus and cytoplasm, implying the presence of dephosphorylated and phosphorylated form of these seven proteins, furthermore, five of them possessed transcription activation activity whereas the left two functioned as transcriptional repressors. Another two members, however, neither localized in the nucleus nor had transactivation activity. Besides,SlBES1.8showed flower-specific expression while other members expressed ubiquitously in all organs. Moreover,SlBES1genes exhibited variational expression in response to nine principal plant hormones. Notably, the expression levels ofSlBES1genes presented a dominant downregulated trend in response to stresses. Conclusions In this study, we systematically analyzed the genomic characterization ofSlBES1family, together with the analyses of protein functional features and expression patterns, our results lay a foundation for the functional research ofSlBES1family.

Funded by the USDA-ARS. Developed by the USDA-ARS SoyBase and Legume Clade Database group at the Iowa State University, Ames, IA

U.S. Public Domain