Reference Report for AP20210624.1
| Title: | Knockdown of GmVQ58 encoding a VQ motif-containing protein enhances soybean resistance to the common cutworm (Spodoptera litura Fabricius) |
| Authors: | Li, X., Qin, R., Du, Q., Cai, L., Hu, D., Du, H., Yang, H., Wang, J., Huang, F., Wang, H., Yu, D. |
| Source: | Li et al. 2020 Journal of Experimental Botany, 71(10):3198-3210 |
| Abstract: | Plants have evolved complex defense mechanisms to withstand insect attack. Identification of plant endogenous insect resistance genes is of great significance for understanding plantherbivore interactions and improving crop insect resistance. Soybean (Glycine max(L.) Merr.) is an important crop that is often attacked by the common cutworm (CCW) (Spodoptera lituraFabricius). In this study, based on our transcriptomic data, the geneGmVQ58, encoding a FxxxVQxxTG (VQ) motif-containing protein, was cloned and characterized. This gene showed the highest expression in the leaves and roots and was up-regulated significantly after CCW attack. Constitutive expression ofGmVQ58rescued the susceptibility of an Arabidopsis mutant to CCW, and interference ofGmVQ58in soybean hairy roots enhanced the resistance to CCW. Furthermore, GmVQ58 was localized to the nucleus and physically interacted with the transcription factor GmWRKY32. The expression of two defense-related genes,GmN:IFRandGmVSP, was up-regulated inGmVQ58-RNAi lines. Additionally, the promoter region ofGmVQ58was likely selected during domestication, resulting in different expression patterns in cultivated soybeans relative to wild soybeans. These results suggest that silencingGmVQ58confers soybean resistance to CCW |
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