| Authors: | Wange, J, Chen, P, Wang, D, Shannon, G, Shi, A, Zeng, A, Orazaly, M |
| Abstract: | Oil is one of the major chemical constituents that affect the quality of soybean [Glycine max (L.) Merr.] products. Soybean seed with high oil content is a valuable source for cooking oil and biodiesel production. The objective of this study was to identify quantitative trait loci (QTL) for oil content to advance soybean breeding efforts. Two F2Ðderived recombinant inbred line (rIL) populations, consisting of 242 and 214 individuals from the cross of r05Ð638 _ r05Ð 1415 (population 1) and r05Ð4256 _ V97Ð1346 (population 2), respectively, were used for QTL mapping. The F2 plants from the mapping popu- lations were genotyped by simple sequence repeat (SSr) and single nucleotide polymor- phism (SNp) markers. Seeds from F2:3, F2:4, and F2:5 lines were tested for oil content using near infrared transmittance technique based on 13% moisture. Six oil QTL regions located on chro- mosomes 5, 6, 14, 15, and 20 were identi ed. Among the QTL identi ed, a major oil QTL (14Ð 40%) on chromosome 20 linked with eight SNp markers was con rmed across genetic popula- tions, locations, and years; this QTL had nega- tive effect on seed protein content. In popula- tion 2, two novel QTL located on chromosomes 6 and 14 accounting for between 11 and 15% and 8 and 15% of the variation in seed oil con- tent, respectively, were detected. These QTL with linked markers can be used for marker- assisted selection for increased seed oil content in soybean breeding programs. |