Title: | SSR Markers Linking to Seed Traits and Total Oil Content in Soybean |
Authors: | Moongkanna, J., Nakasathien, S., Novitzky, W., Kwanyen, P., Sinchaisri, P., Srinives, P. |
Source: | Thai J. Ag. Sci. 2011, 44(4):233-241 |
Abstract: | Soybean is an economic crop used as a main source of vegetable oil. Quality and quantity of oil in soybean seed is important as a raw material determining manufacturing cost. The markers associating with oil content are useful in selection for soybean lines with high oil. The aim of this research was to develop molecular markers linking to genes controlling seed traits and total oil content in soybean. An F2:3 population comprising 186 families was developed from a cross between Pak Chong 2 and Laos 7122. The population was genotyped by 159 polymorphic SSR markers, and seeds were determined for oil content by hexane extraction method. QTL analysis was done by a simple regression method and composite interval mapping. Finally, 138 SSR markers were grouped into 30 linkage groups covering 1,921.1 cM of soybean genome. Twenty- one polymorphic markers remained unlinked. There were 10 QTLs located on linkage groups A1, C2, E, and G that found associating with nodes per plant, seed length, seed width, hundred seed weight and total oil content. |