| Title: | Identification of a novel salt tolerance gene in wild soybean by whole-genome sequencing |
| Authors: | Qi, X., Li, M., Xie, M., Liu, X., Ni, M., Shao, G., Song, C., Yim, A., Tao, Y., Wong, F., Isobe, S., Wong, C., Wong, K., Xu, C., Li, C., Wang, Y., Guan, R., Sun, F., Fan, G., Xio, Z., Zhou, F., Phang, T., Tong, S., Chan, T., Yiu, S., Tabata, S., Wang, J., Xu, X., Lam, H. |
| Source: | Nature Communications (2014) 5:4340 |
| Abstract: | Using a whole-genome-sequencing approach to explore germplasm resources can serve as an important strategy for crop improvement, especially in investigating wild accessions that may contain useful genetic resources that have been lost during the domestication process. Here we sequence and assemble a draft genome of wild soybean and construct a recombinant inbred population for genotyping-by-sequencing and phenotypic analyses to identify multiple QTLs relevant to traits of interest in agriculture. We use a combination of de novo sequencing data from this work and our previous germplasm re-sequencing data to identify a novel ion transporter gene, GmCHX1, and relate its sequence alterations to salt tolerance. Rapid gain-of-function tests show the protective effects of GmCHX1 towards salt stress. This combination of whole-genome de novo sequencing, high-density-marker QTL mapping by re-sequencing and functional analyses can serve as an effective strategy to unveil novel genomic information in wild soybean to facilitate crop improvement. |