| Title: | Identification of a major quantitative trait locus underlying salt tolerance in ÔJidou 12Õ soybean cultivar |
| Authors: | Shi, X., Yan, L., Yang, C., Yan, W., Moseley, D., Wang, T., Liu, B., Di, R., Chen, P., Zhang, M., Yang, Y., Zhao, Q., Li, X., Ai, W., Liu, D., Qi, W., Zhang, M., Yang, C., Liao, H. |
| Source: | BMC Research Notes 2018, 11:95 |
| Abstract: | Background: Identification of the quantitative trait locus (QTL) underlying salt tolerance is a prerequisite for marker assisted selection in the salt-tolerant breeding process. Methods: In this study, the recombinant inbred lines derived from the salt-tolerant elite soybean cultivar ÔJidou 12Õ and the salt-sensitive elite cultivar ÔJi NF 58Õ were used to identify the QTL associated with salt tolerance, using both salt tolerance rating (STR) and leaf chlorophyll content (SPAD) as indicators. Results: A major salt-tolerant QTL, which was flanked by SSR markers GMABAB and Barcsoyssr_03_1421 on chromosome 3, was identified based on single-marker regression, single trait composite interval mapping, and multiple interval mapping analysis. For STR, the LOD ranged from 19.8 to 20.1; R2 ranged from 44.3 to 44.7%; and the additive effect ranged from 0.876 to 0.885 among the three mapping methods. For SPAD, the LOD ranged from 10.6 to 11.0; R2 ranged from 27.0 to 27.6%; and the additive effect ranged from 1.634 to 1.679 among the three mapping methods. Conclusions: In this study, a major QTL conditioning salt tolerance on chromosome 3 was identified. The DNA markers closely associated with the QTLs might be useful in marker-assisted selection for soybean salt tolerance improvement in Huanghuaihai, China. |
| Title: | Characterization of Genetic Basis on Synergistic Interactions between Root Architecture and Biological Nitrogen Fixation in Soybean |
| Authors: | Shi, X., Yan, L., Yang, C., Yan, W., Moseley, D., Wang, T., Liu, B., Di, R., Chen, P., Zhang, M., Yang, Y., Zhao, Q., Li, X., Ai, W., Liu, D., Qi, W., Zhang, M., Yang, C., Liao, H. |
| Source: | Front. Plant Sci. 2018, 8:1466 |
| Abstract: | Soybean [Glycine max (L.) Merr] is an important legume crop and its yield largely depends on root architecture (RA) and biological nitrogen fixation (BNF). However, the relationship between RA and BNF, and its genetics behind remain unclear. Here, two soybean genotypes contrasting in RA and their 175 F9:11 recombinant inbred lines (RILs) were evaluated in field. The shallow-root parent, JD12, had better nodulation and higher yield than the deep-root parent, NF58. Strong correlations between shoot dry weight (SDW) and RA or BNF traits existed in the RILs, and the shallow-root group had more and heavier nodules, as well as higher SDW. After inoculating with rhizobia, roots became shallower and bigger, showing strong synergistic interactions between RA and BNF. In total, 70 QTLs were identified for the 21 tested traits. Among them, qBNF-RA-C2, qBNF-RA-O, and qBNF-RA B1, were newly identified QTLs for BNF and/or RA traits in soybean, which co-located with the QTLs for SDW detected presently, and with the QTLs for yield identified previously. The results together suggest that there are synergistic interactions between RA and BNF, and the QTLs identified here could be used for breeding new soybean varieties with higher yields through optimization of RA traits and BNF capacity. |