| Title: | Identification of Quantitative Trait Loci for Sucrose Content in Soybean Seed |
| Authors: | Zeng, P., Chen, P., Shi, A., Wang, D., Zhang, B., Orazaly, M., Florez-Palacios, L., Brye, K., Song, Q., Cregan, P. |
| Source: | Crop Sci. 2014, 54(2):554-564 |
| Abstract: | Sucrose is a desirable sugar in soybean seed that affects the quality of soyfoods and feed; therefore, soybean cultivars with high sucrose would be valuable for soyfood and soybean meal markets. The objective of this study was to identify quantitative trait loci (QTL) associated with seed sucrose content using simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers that can be used for indirect selection in breeding. A low sucrose line, MFS-553, was crossed with a high sucrose plant introduction, PI 243545, to develop an F2Ğderived QTL mapping population. A total of 626 SSR primers covering 20 soybean chromosomes were used to screen the parents and 209 SSR markers were polymorphic. The polymorphic SSR markers were used to genotype 220 F2:3 lines. Subsequently, a total of 94 F3:4 lines derived from the initial F2:3 population were genotyped with 5361 SNP markers spanning 20 chromosomes, of which 2016 were polymorphic. Seed from the F2:3, F3:5, and F3:6 lines were analyzed for sucrose using high performance liquid chromatography (HPLC). Following the linkage map construction, composite interval mapping (CIM) and multiple interval mapping (MIM) were performed to locate sucrose QTL. Three novel QTLs for seed sucrose were mapped to chromosomes 5, 9, and 16, accounting for 46, 10 and 8%, respectively, of the phenotypic variation for sucrose content. SSR and SNP markers linked to these QTLs can be used for marker assisted selection (MAS) in breeding soybean lines with the desired sugar profile. |