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SoyBase and the Soybean Breeder's Toolbox
Integrating Genetics and Molecular Biology for Soybean Researchers |
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Flanking sequences are returned as 5' -> 3' sequences from the 'top' strand of the genome sequence, i.e. the sequence shown in the SoyBase genome viewer. This has important implications as to where base 1 of the returned sequence actually is with respect to the gene.
The figures below show where base 1 in the returned flanking sequence is located for genes on either the 'top' or 'bottom' strand when 100 bases of both 5' and 3' flanking sequence were requested.
